Expressing:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
Quantitative RT-PCR:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
Recombinant:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
Software:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
Microscopy:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
Derivative Assay:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
Virus:Article Title: MeCP2 regulates telencephalic development in human cerebral organoids.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: MeCP2 +/+ hESC, 2982-WT (p.K82Nfs*43-WT) Trujillo et al. 68 N/A Human: MeCP2 − /− hESC, 2982-MUT (p.K82Nfs*43-MUT) Trujillo et al. 68 N/A Oligonucleotides Primer sequences used for genotyping (Table S2) This study N/A Primer sequences used for allelic expression (Table S3) This study N/A Primer sequences used for RT-qPCR (Table S4) This study N/A Recombinant DNA and viral vectors pLenti-CMV::GFP-WPRE-Puro addgene Cat# 17448 pLenti-Dlxi1/2b:eGFP Gift from Sergiu P. Pas ‚ca and John L. Rubenstein N/A pLenti-Dlxi1/2b:eGFP-WPRE-Puro This study N/A LV-Dlxi1/2b:eGFP VectorBuilder N/A LV-Dlxi1/2b:eGFP-WPRE-Puro VectorBuilder N/A AAV7m8-hsyn1-eGFP Unitat de Producció de Vectors, Universitat Autò noma de Barcelona N/A pAAV-hsyn1-GCaMP6s-P2A-nls-dTomato addgene Cat# 17448 AAV7m8-hsyn1-GCaMP6s-P2A-nlsdTomato VectorBuilder N/A Software and algorithms Adobe Illustrator (CC) Adobe https://www.adobe.com/products/ illustrator.html CellRanger 10X Genomics https://www.10xgenomics.com/support/ software/cell-ranger/latest DESeq2 Bioconductor https://bioconductor.org/packages/ release/bioc/html/DESeq2.html GraphPad Prism GraphPad Software Inc., La Jolla, CA, USA https://www.graphpad.com/features ImageJ (Fiji) ImageJ https://imagej.net/software/fiji/ Imaris Oxford Instruments https://imaris.oxinst.com R R Core Team https://www.r-project.org/ RStudio Posit https://posit.co/download/ rstudio-desktop/ Seurat Satija Lab https://satijalab.org/seurat/ Other 6-well plate for iPSC culture Corning Cat# 3516 96-well glass bottom microplate ibidi Cat# 89627 Aggrewell TM 800 plate StemCell Technologies Cat# 34815 Fisherbrand TM Superfrost TM Plus microscope slides FisherScientific Cat# 12-550-15 Ultra-low attachment 6-well plate Corning Cat# 3471 24 Cell Reports 44, 116670, December 23, 2025 Experimental cell lines: RTT hPSC lines RTT hiPSCs were obtained from the Rett Syndrome Research Trust (RSRT) iPSC Collection deposited at the Coriell Institute for Medical Research and were derived from blood lymphocytes and reprogrammed at the Harvard Stem Cell Institute with Sendai virus vectors (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Invitrogen, A16518). ..
|
Addgene inc is a verified supplier 